Learn CUPL with Cupl Software: A Comprehensive Tutorial

PCR products were prepared for capillary electrophoresis (CE) by combining 1 µl of amplified product with 9 µl of a 30:1 mixture of deionized Hi-Di formamide (Applied Biosystems, Foster City, USA) and Internal Lane Standard ORG (Peoplespot Inc., Beijing, China). PCR products were separated and detected on an ABI PRISM 3500xL Genetic Analyzer (Applied Biosystems, Foster City, USA) and analyzed by GeneMapper ID v. 1.4 software (Applied Biosystems, Foster City, USA) according t o the manufacturer's instructions. Control DNA 007 (Applied Biosystems, Foster City, USA) or 9948 (Applied Biosystems, Foster City, USA) were genotyped as a standard reference.

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